Figure 8. Angles and contacts across all phosphates: Scissile phosphate is unique in α, β, γ, and δ catalysis.
(A) In-line attack angles for scissile (filled bars) and non-scissile (open bars) phosphates in the glmS (pink), hammerhead (orange), twister (teal), and hairpin (blue), ribozymes (α strategy). (B,C,D) Number of contacts within 5 Å to the (B) O2′ (γ and γ′ strategy), (C) NBO atoms (β and γ′′ strategy), and (D) O5′ (δ strategy) at scissile (filled bars) and non-scissile (open bars) phosphates. Ribozyme coloring is the same as for (A).