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Glutathione S-transferase–sialic acid binding protein-1 (GST-SABP1) specifically bound to sialic acid in vitro. A, Evaluation of the binding specificity between SABP1 and sialic acid by western blot assay. GST-SABP1 bound to the sialic acid-agarose beads but not to the agarose. GST did not bind to sialic acid-agarose beads. B, Binding of GST-SABP1 to the sialic acid-agarose beads was inhibited by preincubation of the recombinant protein with sialic acid.
