Figure 3.

Recombinant sialic acid binding protein-1 (SABP1) bound to both Vero and dendritic cells in vitro and inhibited Toxoplasma gondii invasion. A, Western blot analysis of cell adhesion in vitro. Glutathione S-transferase (GST)-SABP1 fusion protein and GST protein were incubated with Vero and dendritic cells (DC). GST-SABP1 bound to the cells but the GST protein did not. B, Indirect immunofluorescence assay showed a specific interaction between GST-SABP1 fusion protein and host cells (green). C, Preincubation of GST-SABP1 fusion protein with host cells significantly inhibited parasite invasion. Error bars indicate the mean ± SD (n = 3). *** P < .001. Abbreviation: DAPI, 4′,6-diamidino-2-phenylindole.