Figure 4.

Binding of glutathione S-transferase–sialic acid binding protein-1 (GST-SABP1) to host cells was sensitive to neuraminidase treatment. Western blot analysis showing the effect of neuraminidase treatment on the binding of GST-SABP1 to Vero (A) and dendritic cells (B). Vero and dendritic cells were treated with neuraminidase before incubation with GST-ABP1 protein. C and D, Binding of GST-SABP1 to Vero and dendritic cells was gradually abolished with increased neuraminidase concentration; GST-SABP1 protein levels detected on (C) Vero and (D) dendritic cells surface. PE-A: the red fluorescence signal detected in PE channel by flow cytometer (PE: phycoerythrin, A: area).