Fig. 3.

Changes in B cell subsets in thawed PBMC. a FACS gating strategy used to identify CD19+20+CD27+ total memory B cells, CD19+20+ total naïve B cells, and CD19+ CD20+CD27-CD24^hiCD38^hi B cells. b Total CD19+CD20+CD27+ memory B cells. c CD19+CD20+CD27- total naïve B cells. d CD19+CD20+CD27-CD24^hiCD38^hi B cells. e Ratio of total memory B cells to naïve “regulatory” CD24^hiCD38^hi B cells. f Ratio of total naïve B cells to CD19+CD20+CD27-CD24^hiCD38^hi B cells. Data represent median/IQR values for all patients at each timepoint; open circles indicate data points for each individual patient. p values indicate significant changes from baseline (M0) values as follows: ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05; Kruskal-Wallis test