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. 2020 Jun 11;15:4125–4138. doi: 10.2147/IJN.S253125

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© 2020 Ou et al.

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Overexpression of TLR4 on macrophage membranes results in increased inhibition of LPS-mediated macrophage activation. Fluorescence microscopy observations of the transfection efficiency of plasmid transfection for 72 h (Scale bar: ×200) (A). Western blotting was used to detect the transfection efficiency (B). qRT-PCR was used to detect the transfection efficiency (C). Western blotting was used to detect the protein expression of MyD88, IRAK1, p-p65 and p65 (D). ELISA was used to detect the remaining LPS in each group (E). ELISA was used to detect the remaining LPS in different concentrations of M-NPs and TLR4+/M-NPs (F). *p < 0.05, **p < 0.01, ***p < 0.001.