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. 2020 Jun 11;14:2335–2353. doi: 10.2147/DDDT.S249093

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© 2020 Hassan et al.

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Hepatoprotective effect of Ganoderma lucidum on immunohistochemical expression of Bcl-2 in rat livers. (1) Representative microscopic pictures of liver sections immunostained using Bcl-2 antibody, showing mild positive staining score (1) in the control group (A and B), GL group (C and D). Negative staining score (0) in CP group (E and F), modulation of Bcl-2 as mild positive staining score (1) in i.p group (G, H), moderate positive staining score (2) in EOD group (I and J), and strong positive staining score (3) in daily-treated group (K and L). Black arrows point to positive staining. X: 100 bar 100 (A, C, E, G, I, and L) and X: 400 bar 50 (B, D, F, H, and J). IHC counterstained with Mayer’s hematoxylin. (2) Statistical analysis of IHC staining intensity scores in six experimental groups showing a significant increase in Bcl-2 in daily-treated group when compared with all groups. Different small alphabetical letters means significant when P< 0.05. ^aSignificant against control group; ^bsignificant against GL group; ^csignificant against CP group; ^dsignificant against i.p. group; ^esignificant against EOD group.