Table 1.
Primer sequences of RT-PCR in colon tissues.
| Gene | Sequence (5′-3′) |
|---|---|
| TNF-α | F: AAGCCTGTAGCCCACGTCGTA |
| R: AGGTACAACCCATCGGCTGG | |
| IL-1β | F: GGA GAC TTC ACA GAG GAT AC |
| R: CCA GTT TGG TAG CAT CCA TC | |
| IL-6 | F: TCC ATC CAG TTG CCT TCT TG |
| R: TTC CAC GAT TTC CCA GAG AAC | |
| CSF-1 | F: GGCTTGGCGGGATGATTCT |
| R: GAGGGTCTGGCAGGTACTC | |
| MCP-1 | F: GAGGACAGATGTGGTGGGTTT |
| R: AGGAGTCAACTCAGCTTTCTCTT | |
| IL-10 | F: GCT CTT ACT GAC TGG CAT GAG |
| R: CGC AGC TCT AGG AGC ATG TG |
RT-PCR – reverse transcription polymerase chain reaction; TNF – tumor necrosis factor; IL – interleukin; CSF-1 – colony stimulating factor 1; MCP-1 – monocyte chemoattractant protein-1.