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. Author manuscript; available in PMC: 2021 Apr 16.
Published in final edited form as: Cell. 2020 Mar 18;181(2):460–474.e14. doi: 10.1016/j.cell.2020.02.049

Figure 4. Protein Complexes Validated by Calibrated Molecular Mass Determination and Direct Chemical Cross-linking.

Figure 4.

(A) Observed mass vs. predicted monomeric mass in a representative Arabidopsis size exclusion chromatography (SEC) fractionation. Shading reflects the number of orthogroups per hexagonal bin.

(B) Cross-linked proteins from soy and Chlamydomonas are more likely (green line, log-likelihood) to have high CF-MS scores compared to non-cross-linked observed proteins.

(C-D) Inter-subunit cross-links only appear in fractions where complex subunits co-elute. Elution profile and inter-subunit crosslinks for soy T-complex chaperonin (CCT) shown in (C) and Chlamydomonas Photosystem II (PSB) shown in (D)

(E-F) 3D homology models of complexes (see STAR methods) with observed inter-subunit cross-links (black lines). Soy CCT (E) is colored by subunit, and Chlamydomonas

Photosystem II (E) highlights PSBB, PSBC, and PSBO as blue, red, and yellow, respectively.