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. 2020 Jun 5;8:529. doi: 10.3389/fbioe.2020.00529

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Copyright © 2020 Paoletti, Divieto, Tarricone, Di Meglio, Nurzynska and Chiono.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miRcombo-transfected cells exhibit calcium transients. (A,B) Representative image, recorded with fluorescence microscopy (A) and trace (B) of Ca⁺² oscillations in AHCFs transfected with miRcombo (n = 3) and negmiR (n = 3) after 30 days from transfection. Scale bar: 25 μm. (C) Bar graph reports the percentage of cells showing Ca⁺² transients after 30 days from miRcombo and negmiR transfection. Stated P-value is versus negmiR.