Fig. 1.

The PEDV genome was modified by m⁶A methylation.

The PEDV genome was extracted from purified viral particles and used for m⁶A quantification and m⁶A-seq. A) Purified PEDV particles were observed by transmissible electron microscope analysis. Scale bar, 200 nm. B) The m⁶A level of PEDV RNA was quantified by ELISA. The synthetic scrambled RNA was employed as a negative control. C) Map of m⁶A reads on the PEDV genome. The red line represents m⁶A-seq, and the blue line indicates input RNA-seq. The red bars of the PEDV genome indicate m⁶A peaks identified in duplicate experiments by MeRIPPeR analysis (p < 0.05). The blue bars indicate the fragment of the PEDV genome without m⁶A modification.