Fig. 2.

PEDV replication was regulated by m⁶A modification.

The siRNAs against porcine METTL3/METTL14/FTO were transfected into different cells and the cells were infected with PEDV at a MOI = 0.1. The supernatants were collected at different time points for viral titration. A) Western blotting analysis of LLC-PK1 cells transfected with siRNAs and scrambled siRNA as the negative control (NC) at 48 hpi. B) The relative fold change of viral titers in LLC-PK1 cells transfected with siRNAs or NC at different time points. C) The relative fold change of viral RNA copies of PEDV in LLC-PK1 cells at different time points. D) The relative fold change of viral titers in LLC-PK1 cells overexpressing FTO at different time points. E) Western blotting analysis of Vero cells transfected with siRNAs or NC at 48 hpi. F) The relative fold change of viral titers in Vero cells transfected with siRNAs or NC at different time points. G) The relative fold change of viral RNA copies of PEDV in Vero cells at different time points. H) The relative fold change of viral titers in Vero cells overexpressing FTO at different time points. I) Western blotting analysis of constructed cell lines with METTL3/METTL14 knockdown and with GFP-FTO overexpressed. J) The m⁶A level of PEDV RNA produced in different cell lines was quantified by ELISA. The synthetic scrambled RNA was taken as a negative control. K) The growth curve of PEDV derived from different cell lines. *P < 0.05, **P < 0.01, ns means not significant.