Fig. 3.

YTHDF 1–3 proteins negatively regulated PEDV replication.

Cells were transfected with Myc-YTHDF1-3 and infected with PEDV at a MOI = 0.1. The cells were then lysed and used for RNA-binding analysis 24 hpi. A) Real-time PCR analysis of PEDV genome binding to YTHDF1-3 proteins in LLC-PK1 cells. EV represents the empty vector. B) Real-time PCR analysis of PEDV genome binding to YTHDF1-3 proteins in Vero cells. C) Western blotting analysis of LLC-PK1 cells transfected with siRNAs and NC at 48 hpi. D) The relative fold change of viral titers in LLC-PK1 cells transfected with siRNAs or NC at different time points. E) The relative fold change of viral titers in LLC-PK1 cells transfected with myc-YTHDFs or EV at different time points. F) Western blotting analysis of Vero cells transfected with siRNAs or NC at 48 hpi. G) The relative fold change of viral titers in Vero cells transfected with siRNAs or NC at different time points. H) The relative fold change of viral titers in Vero cells transfected with myc-YTHDFs or EV at different time points. I) Effect of YTHDF2 knockdown combined with Actinomycin D (Act-D) treatment on the expression and stability of mRNA in LLC-PK1 cells. J) Effect of YTHDF2 knockdown combined with Act-D treatment on the expression and stability of mRNA in Vero cells. *P < 0.05, **P < 0.01.