Fig. 6.

M⁶A modification regulates the host response to PEDV infection

A) The pathway network analysis of different signaling pathways in which the m⁶A-modified genes were involved. B) Real-time PCR verification of m⁶A-modified genes involved in the tight junction and MAPK/p53 signaling pathway. C) Real-time PCR analysis of selected m⁶A-modified genes in LLC-PK1 cells. The expression levels of all genes were normalized to GAPDH levels (internal control). The 2^−ΔΔCt method was used to calculate the relative gene expression data. All experiments were performed in triplicate.