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. 2020 Jun 16;16(6):e1008538. doi: 10.1371/journal.ppat.1008538

Fig 2. Peli1 promotes ZIKV infection and induction of innate immunity in macrophages.

Fig 2

A- D. WT and Peli1-/- mice were infected with ZIKV-FSS13025. A. Viral load in the spleen at day 2 pi and non-infected (NF) mice was determined by qPCR assay. Data are presented as mean ± SEM, n = 5–8. B-D. Blood cytokine levels at day 2 were determined by Bioplex. Data are presented as the means ± SEM of 4–5 samples from 2 independent experiments. E-F. Viral load of ZIKV-FSS13025 -infected macrophages (MOI 0.5) and DCs (MOI 5) was measured by qPCR assays. n = 5–6 per group. G-H. Cytokine RNA levels of DCs were measured at indicated time points by qPCR. Data represent means ± SEM of 5–6 samples. I-L. WT and Peli1-/- macrophages were blocked with (MAR1-5A3, 25ug/ ml) followed by ZIKV-FSS13025 infection (MOI = 5). Viral load (I) and cytokine RNA levels (J-L) were measured at day 4 pi by qPCR. Data are presented as the fold increase compared to mock- infected (means ± SEM), n = 6. ** P < 0.01 or *P < 0.05 compared to WT group (Unpaired t test).