Figure 1.

Effect of empagliflozin on expression of HIF-1α pathway in HK-2 cell line. HK-2 cells were planted in 5.5mM glucose and 25mM glucose medium with or without empagliflozin treatment under normoxia condition for 72 h. (A) Western blotting was performed using anti-HIF-1α, anti-GLUT-1 and anti-β-actin antibodies. β-actin was used as a loading control. (B, C) Quantification of the Western blot data. (D–E) Dot plots scatter represented individual measurement. The data represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 vs. high glucose.

Abbreviation: ns, non-significant.