Figure 2.

Effect of empagliflozin on SGLT2 expression in HK-2 cells. (A) Representative of Western blots illustrating SGLT2 and β-actin expression. HK-2 cells were planted in 5.5mM glucose and 25mM glucose medium with or without empagliflozin treatment for 72 h. Western blotting was performed using anti- SGLT2 and anti-β-actin antibodies. β-actin used as a loading control. (B) Quantification of Western blot data in HK-2 cells. (C) Dot plots scatter represented individual measurement. The data represent the mean ± SD of three independent experiments.*P < 0.05, ***P < 0.001, ****P < 0.0001 vs. high glucose.

Abbreviation: ns, non-significant.