Figure 3.

Effect of high glucose on expression of proteins that affect fibrosis in HK-2 cell line. HK-2 cells were planted in 5.5mM glucose, 5.5mM glucose plus 20mM mannitol and 25mM glucose for 24 h. (A) Western blotting was performed using anti-fibronectin, anti-collagen IV, anti-TGF-β1, and anti-β-actin antibodies. β-actin used as a loading control. (B–D) Quantification of the Western blot data in HK-2 cells. (E–G) Scatter dot plots represented individual measurement. The data represents the mean ± SEM of three independent experiments. *P < 0.05, ****P < 0.0001 vs. high glucose.

Abbreviation: ns, non-significant.