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. 2020 Jun 16;15(6):e0234696. doi: 10.1371/journal.pone.0234696

Fig 4. Increase of the detected poly(A) tail lengths by sequencing the larger size of cDNA library and by reducing the size of RNA fragments.

Fig 4

A. Electrophoresis gel images of selected cDNA libraries for Poly(A)-seq in two benches of experiments. In experiment 1, after size selection, we separated two different selection products on an agarose gel. “Large” and “Small” were significantly different in sizes, indicating the inserted RNA fragments were not homogenized. In experiment 2, no much difference between these two selections was observed, indicating the inserted RNA fragments were homogenized and smaller. B. Distribution of poly(A) tail length in poly(A)-containing reads in Large and Small cDNA libraries in the experiment 1. C. Poly(A) tail length distribution in reads across all samples. D. Distribution of median poly(A) tail lengths in all genes across all samples.