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. 2020 Jun 16;15(6):e0234696. doi: 10.1371/journal.pone.0234696

Fig 5. Hire-PAT PCR assay of poly(A) tail length obtained by Poly(A)-seq method.

Fig 5

A. Poly(A) tail length validation of the 40-nt spike-in using Hire-PAT PCR assay. PCR product was showed in top panel and the experiment was characterized in the bottom panel. B. Poly(A) tail length of NPM1, ACTB, and GAPDH mRNAs measured by PCR-based method (Hire-PAT) in HeLa cell and HCT116 cell. Gene-specific forward and reverse primers (S) amplify the region just upstream (<10bp) of poly(A) tails of target genes (top-left).