Table 2. Poly(A)-seq profiles of human cells.

Sample	Total reads	Total genes	Median poly(A) length	Sequencing platform	Run
Bench 1
HeLa_B1_R1	11292091	13490	60	Nextseq 500	1,2
HeLa_B1_R2	11805400	13306	63		1,2
Bench 2
HeLa_B2_R1	12846233	15656	93	Nextseq 500	3,4
HeLa_B2_R2	8879440	14482	98		3,4
HCT116_B2_R1	10695232	14761	101		3,4
HCT116_B2_R2	12543975	14403	102		3,4
HEK293T_B2_R1	14640822	16487	100		3,4
HEK293T_B2_R2	13729515	16240	103		3,4
Spike-in
HeLa_1.A40	20935	spike-in	44	HiSeq X Ten	5
HeLa_2.A40	6478	spike-in	44		5
HeLa_1.A120	85531	spike-in	105*		5
HeLa_2.A120	23827	spike-in	105*		5
HeLa_1.A40	21821	spike-in	42	NovaSeq 6000	6
HeLa_2.A40	7815	spike-in	42		6
HeLa_1.A120	59179	spike-in	112		6
HeLa_2.A120	18404	spike-in	109		6
Cells
HeLa_1	5303467	11352	67	HiSeq X Ten	5
HeLa_2	6246736	11300	65		5
HeLa_1	8419838	11950	54	NovaSeq 6000	6
HeLa_2	10385586	12015	53		6

Total reads: Total number of uniquely mapped reads that contain poly(A) tails > = 10 nt.

Total genes: Total number of genes with > = 1 poly(A)-containing reads.

% 3’ adaptor reads: Percentage of total reads that contain 3’ adaptor sequences downstream of poly(A) regions.

Median poly(A) length: Median poly(A) length in total reads.

“B*” in sample name means different bench. “R*” means replicates.