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. 2020 Jun 12;13:5467–5478. doi: 10.2147/OTT.S236514

Figure 4.

Figure 4

miR-423 activates the NF-κB pathway through regulation of TNIP2. (A) GSEA plot showing miR-423 expression was positively correlated with NF-κB-induced gene signature and AKT-activated gene signature, and inversely correlated with NF-κB-suppressed gene signature in TCGA BRCA cohorts. (B) Luciferase activity of NF-κB activity in indicated cells. (C) Western blot analysis of snail1 and twist1 expression in indicated cells, α-Tubulin was used as loading control. (D) Luciferase activity of NF-κB activity in miR-423 overexpressing cells with transfected the plasmids of vector or IκBα mutation. (E) Western blot analysis of snail and twist expression in miR-423 overexpressing cells with transfected the plasmids of vector or IκBα mutation, α-Tubulin was used as loading control. (F) Transwell assays showed the invasiveness of MCF-7 cells was inhibited in miR-423-overexpressing cells after transfected with IκBα mutation. Bars represent the mean ± SD of three independent experiments; **P < 0.01,*P < 0.05.