(a) Sketch of the horizontal view of the mouse brain with location of cortical datasets used for comparison. See also Figure 1a. (b,c) Comparative analysis across cortical regions. Postsynaptic target specificity for axons seeded from (b) layer 2 ADs (n = 21, 20, 21, 30 for S1, V2, PPC and ACC, respectively) and (c) for axons seeded from deep layer ADs (n = 19, 20, 20, 32 for S1, V2, PPC and ACC, respectively). Note the high level of quantitative consistency of synaptic target fractions across cortices with the exception of somatic innervation in axons seeded from DL ADs. Error bars indicate mean ± SEM. *p < 0.05 MANOVA test followed by multiple One-way ANOVA with Bonferroni correction.