. 2020 Mar 14;66(3):281–286. doi: 10.1262/jrd.2019-156

Table 2. Effect of precocious activation prevention on development to blastocysts of somatic cell nuclear transfer (SCNT) oocytes.

	No. of embryos	No. of cleaved embryos (%)	No. of blastocysts (%)	Cell no. in blastocysts
Control	164	148 (90.2 ± 2.8) ^a	19 (11.6 ± 0.8) ^a	49.1 ± 2.8 ^a
Precocious activation prevention	289	283 (97.9 ± 1.1) ^b	68 (23.5 ± 6.8) ^b	65.0 ± 3.0 ^b

Precocious activation prevention: SCNT runs in which oocytes were maintained at metaphase II (M-II) stage for 18 h, then fused with single cells in low-Ca²⁺ fusion medium, before incubated in medium supplemented with cytochalasin throughout the SCNT after fusion steps. Three replications were performed. ^{a, b} Superscript letters denote significant difference (P < 0.05) in the same column.