Table 3. Developmental competence of somatic cell nuclear transfer (SCNT) embryos derived from cells serum-starved by different methods.
| No. of embryos | No. of cleaved embryos (%) | No. of blastocysts (%) | Cell no. in blastocysts | ||
|---|---|---|---|---|---|
| Fibroblasts | No-serum | 175 | 170 (97.1 ± 1.4) | 56 (32.0 ± 7.7) | 86.8 ± 5.9 |
| Low-serum | 188 | 184 (97.9 ± 1.2) | 33 (17.6 ± 7.9) | 79.9 ± 5.2 | |
| Cumulus cells | No-serum | 102 | 99 (96.0 ± 3.5) a | 21 (20.6 ± 3.5) | 60.9 ± 5.6 |
| Fresh (no starvation) | 102 | 90 (88.2 ± 1.4) b | 24 (23.5 ± 3.5) | 50.0 ± 8.6 | |
No-serum: cells were serum-starved in fetal bovine serum (FBS)-free DMEM for 2 days before used for nuclear transfer. Low-serum: cells were serum-starved in 0.5% FBS DMEM for 5 days before used for nuclear transfer. Three replications were performed. a, b Superscript letters denote significant difference in the same column.