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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Hepatology. 2020 May 8;72(2):609–625. doi: 10.1002/hep.31041

Fig. 6. Ablation of TLR3 in Kupffer cells ameliorates liver injury in chronic and acute alcohol consumption.

Fig. 6.

(A) After 8 weeks of WT bone marrow (BM) transplantation to WT mice and TLR3 KO mice, chimerism of Kupffer cells, BMs, or PBMCs was assessed by PCR. After confirmation of chimerism, the mice were fed a liquid EtOH diet for 10 days (E10d), and 1 binge drinking (4 g/kg) (n = 5/group). (B) Serum levels of ALT, AST, TG and TC were measured. (C) Liver sections were stained with H & E. Bar = 50 μm. (D, E) IL-17A expressing T, Ly6G+CD11b+ and F4/80+CD11b+ cells of liver MNCs were analyzed by flow cytometry after chronic and binge ethanol consumption. (F) Liver MNCs were subjected to qRT-PCR analyses (3 replicates). Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01 compared to the corresponding control, based on unpaired t-test between two groups and one way ANOVA with Dunnett’s test for multiple comparison vs control.