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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Hepatology. 2020 May 8;72(2):609–625. doi: 10.1002/hep.31041

Fig. 8. RNA sequencing reveals increased mitochondrial RNA contents in exosomes of ethanol-treated human hepatocytes.

Fig. 8.

Human hepatocytes were treated with or without 100 mM ethanol for 24 hours, following which hepatic exosomes were isolated from media. (A) Size distribution and total RNA content isolated from hepatic exosomes were assessed by electrophoresis (3 replicates). (B) Distribution of the mitochondrial gene transcripts from Veh-Exo RNA (left) or EtOH-Exo RNA (right) sequencing. (C,D) Distribution of the mitochondrial gene transcripts (chrM) in the top 50 RNA from EtOH-Exo RNA sequencing, and their relative expression compared to those of Veh-Exo RNA. (D) Heavy (outer circle)- and light (inner circle)-stranded location of increased mitochondrial gene transcripts (blue-colored arrow) from EtOH-Exo RNA sequencing. (E) Interaction mechanisms between mtdsRNA of hepatocytes and TLR3 in Kupffer cells mediated by exosomes.