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Conceptual design of the 3D printed BrCa bone metastatic models. (a) Beam-scanning SL 3D printing of BrCa bone model. (b) Schematic 3D view of the triculture model. (c) Schematic of the in vivo invasion of MDA-MB-231 cells into bone, and 2D view of our triculture model. The localized niche has three neighboring regions, consisting of microvascularized bone, endothelial vessels, and BrCa cells. The specific features of the model are also highlighted in the tan shaded area.
