Fig. 3.

CU06-1004 reduces increased AQP4 expression after I/R. a Comparison of AQP4 expression in the sham, vehicle-I/R, and CU06-1004-I/R groups. Square: enlarged image of the region. Arrows show the presence of AQP4 on astrocytes. b Quantification of AQP4-positive astrocytes. n = 5 per group; scale bars:, 20 μm. c Effects of CU06-1004 administration on AQP4 expression in brain lysates. d, e Quantification of blots using ImageJ software. n = 3 independent experiments. f Effects of CU06-1004 administration on EAAT1/2 expression in brain lysates. g−j Quantification of blots using ImageJ software. n = 3 independent experiments. ***P < 0.001. Error bars indicate mean ± SEM. ns, not significant; DAPI, 4′,6-diamidino-2-phenylindole; AQP4, aquaporin 4; GFAP, glial fibrillary acidic protein; I, ipsilateral hemisphere; C, contralateral hemisphere; 1004, CU06-1004