Fig. 4.

CU06-1004 alleviates increased CX43 expression after I/R. Comparison of CX43 expression in the sham, vehicle-I/R, and CU06-1004-I/R groups (a, c). Square, enlarged image of the region. Quantification of CX43-positive astrocytes (b) and blood vessels (d). n = 5 per group; scale bars, 20 μm. Effects of CU06–1004 administration on CX43 expression in brain lysates (e). Quantification of blots using ImageJ software (f, g). n = 3 independent experiments. ***P < 0.001. Error bars indicate mean ± SEM. ns, not significant; DAPI, 4′,6-diamidino-2-phenylindole; CX43, connexin 43; GFAP, glial fibrillary acidic protein; CD31, cluster of differentiation 31; I, ipsilateral hemisphere; C, contralateral hemisphere; 1004, CU06-1004