Fig. 5.

CU06-1004 prevents the disruption of basal membrane proteins in ECs after I/R. Comparison of β1-integrin (a) and β-DG (c) expression in the sham, vehicle-I/R, and CU06-1004-I/R groups. Square, enlarged image of the region. Quantification of β1-integrin- (b) and β-DG- positive (d) blood vessels. n = 5 per group; scale bars, 20 μm. Effects of CU06–1004 administration on β1-integrin and β-DG expression in brain lysates (e). Quantification of blots using ImageJ software (f−i). n = 3 independent experiments. ***P < 0.001. Error bars indicate mean ± SEM. ns, not significant; DAPI, 4′,6-diamidino-2-phenylindole; DG, dystroglycan; CD31, cluster of differentiation 31; I, ipsilateral hemisphere; C, contralateral hemisphere; 1004, CU06-1004