Fig. 6.

CU06-1004 inhibits the reduction in extracellular matrix proteins after I/R. Comparison of LAM (a) and COLIV (c) expression in the sham, vehicle-I/R, and CU06-1004-I/R groups. Square, enlarged image of the region. Quantification of LAM- (b) and COLIV-positive (d) blood vessels. n = 5 per group; scale bars, 20 μm. Effects of CU06-1004 administration on LAM and COLIV expression in brain lysates (e). Quantification of blots using ImageJ software (f−i). n = 3 independent experiments. ***P < 0.001. Error bars indicate mean ± SEM. ns, not significant; DAPI, 4′,6-diamidino-2-phenylindole; LAM, laminin; COLIV, collagen type IV; CD31, cluster of differentiation 31; I, ipsilateral hemisphere; C, contralateral hemisphere; 1004, CU06-1004