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. 2020 Jun 16;10:9704. doi: 10.1038/s41598-020-66539-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of characteristics of Bcl6-LKO mice fed with CDAHFD for 38 weeks. (a) Body weight and liver weight were measured. The percent weight of liver relative to the body (% body weight of liver) weight was calculated. The serum levels of triglyceride (TG), total cholesterol, and high-density lipoprotein (HDL) cholesterol levels were measured. Results are represented as mean ± standard deviation (S.D.) (n = 15 for male wild-type mice, n = 8 for female wild-type mice, n = 17 for male Bcl6-LKO mice, n = 11 for female Bcl6-LKO mice). (b) The serum levels of glutamic pyruvic transaminase (GPT) were measured as an indicator of liver injury. Results are represented as mean ± S.D. (n = 15 for male wild-type mice, n = 8 for female wild-type mice, n = 17 for male Bcl6-LKO mice, n = 11 for female Bcl6-LKO mice). (c) The mRNA expression levels of Tnfα mRNA in the liver were measured by quantitative real-time polymerase chain reaction. The Hprt gene was used as an internal control. The expression of genes in male wild-type mouse livers was set to 1.0. (n = 10 for male wild-type mice, n = 6 for female wild-type mice, n = 10 for male Bcl6-LKO mice, n = 10 for female Bcl6-LKO mice). *P < 0.05, **P < 0.01. MWT, male wild-type mouse samples; FWT, female wild-type mouse samples; MLKO, male Bcl6-LKO mouse samples; FLKO, female Bcl6-LKO mouse samples.