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. 2020 Jun 16;11(6):466. doi: 10.1038/s41419-020-2671-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Heatmap shows cells invasion-related genes regulated by KLF5 knockdown in C4-2 sublines (C4-2-NC/C4-2-shKLF5) detected by high throughput sequencing (RNA-seq). b–e Effect of KLF5 knockdown on the expression of 14 invasion-associated genes in C4-2, 22RV1, PC-3, and DU145 cells, as detected by RT-qPCR. 18S was used as an internal loading control. f, g Western blotting of AR and MMP9 in C4-2 and 22RV1 AR-positive cell lines transfected with shKLF5 or shNC. h, i Western blotting of MMP2 and MMP9 in DU145 and PC-3 in AR-negative cell lines transfected with shKLF5 or shNC. j Analysis of linear correlation between KLF5 and MMP9 from GSE60329. k Analysis of linear correlation between KLF5 and MMP2 from GSE60329.