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. 2020 Jun 16;11:3061. doi: 10.1038/s41467-020-16961-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Clustered heat map of median percent wild-type frameshifting of variants, in which the indicated region (upstream, slippery site (including the preceding codon) and downstream) differs from the native one (n = 37–325). b The median percent wild-type PRF signal of variants, in which the indicated position is changed is plotted for the entire length of the variable region; gray box: slippery site. c Percent wild-type −1 PRF signal for variants with a point mutation only in the indicated position of the slippery site; the box shows the quartiles of the dataset, while the whiskers show the rest of the distribution except for outliers (n = 22, 11, 7, 6, 7, 8, 10, and 17 sequences tested, in the order they appear on the graph). d Heat map showing median percent GFP fluorescence conferred by replacing the native slippery site in −1 PRF sites tested positive in the assay (Fig. 1c), with the indicated combination of slippery site elements. e Distribution of percent wild-type GFP fluorescence of variants, in which the slippery site was replaced by any possible combination of bases following the pattern XXXYYYZ; the box shows the quartiles of the dataset, while the whiskers show the rest of the distribution except for outliers (n = 80, 49, 57, 48, 42, 19, and 56 sequences tested, in the order they appear on the graph). f Distribution of normalized reads across GFP (+1 frame) expression bins for variants of the OAZ1 frameshifting site, in which either the first (left) or the second (right) codon is replaced; blue lines: wild type; green and magenta lines: bin profile for the variant, in which the native codon was replaced by the indicated one; gray lines: bin profiles for other codons at this position. g Percent GFP fluorescence for variants, in which the slippery site was replaced with all possible combinations of the slippery site pattern found in west nile virus (XYYZZZZ); the box shows the quartiles of the dataset, while the whiskers show the rest of the distribution except for outliers (n = 54, 56, 51, 24, 16, and 22 sequences tested, in the order they appear on the graph).