Fig. 6.

Effect of NGR-Dau conjugates 1 and 2 (10 mg/kg Dau content, 7 treatments, black arrows) and free Dau (1 mg/kg, 3 treatments, red arrows) on orthotopic HT-29 human colon carcinoma bearing mice. a Animal body weight (g, average ± SEM). b Tumor weight (g, average ± SEM). c Liver/body weight ratio (percentage, average ± SEM). d Weight of metastases near the primary tumor (g, average ± SEM). Control and groups 1 and 2 were measured after the termination of the experiment (on day 27 after transplantation), while Dau group was measured on day 24 subsequent to transplantation. 8 animals were used per group. Statistical analysis was performed by Mann-Whitney test. *, ** and *** mean significantly different at p < 0.05, p < 0.01, and p < 0.001, respectively