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. 2020 Jun 10;11:1243. doi: 10.3389/fmicb.2020.01243

FIGURE 6.

FIGURE 6

Effects of the C85S mutation on viral infection and viral accumulation in TYLCV-inoculated tomato plants. (A) Symptoms in plants that were agroinoculated with wt TYLCV or TYLCV-C85S at 33 dpi. Mock represents mock-inoculated plants. Enlarged images show the yellowing and curling leaves. (B) The time course of wt TYLCV or TYLCV-C85S infection. Values representing percentages of systemically infected plants at different dpi are given as mean ± SD of triplicate experiments. In each experiment, 15 plants were inoculated and four independent repeats were performed. (C) The aboveground plant heights of Mock-, TYLCV-, or TYLCV-C85S-inoculated tomato plants as measured at 33 dpi. (D) The accumulated viral DNA in plants as measured by qPCR. Accumulated levels of viral DNA were tested in TYLCV-, TYLCV- C85S-, or mock-inoculated plants at 3, 13, 23, and 33 dpi. Total DNA was extracted from newly emerged systemic leaves. Values represent the mean relative to the Mock-treated plants (n = 3 biological replicates) and were normalized with SlActin as an internal reference.