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. 2020 Jun 10;11:1243. doi: 10.3389/fmicb.2020.01243

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Copyright © 2020 Zhao, Wu, Barton, Fan, Ji, Wang and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of the C85S mutation on viral infection and viral accumulation in TYLCV-inoculated N. benthamiana. (A) Symptoms in plants that were agroinoculated with wt TYLCV or TYLCV-C85S at 33 dpi. Mock represents mock-inoculated plants. (B) The time course of wt TYLCV or TYLCV-C85S infection. Values represent percentages of systemically infected plants as performed in Figure 6B. (C) The aboveground plant heights of mock-, TYLCV-, or TYLCV-C85S-inoculated N. benthamiana plants as measured at 33 dpi. (D) The accumulated viral DNA in plants was measured by qPCR. qPCR was done as Figure 6D. NbActin as an internal reference.