FIGURE 9.

Effect of anti-TNF-α neutralizing antibody on PAF C-16 induced intracellular M. smegmatis growth inhibition. M. smegmatis infected THP-1 cells were treated for 24 h with solvent control (10 μl ethanol/ml), anti-TNFα antibody (10 μg/ml), PAF C-16 (1 μg/ml), a combination of isotype control antibody (10 μg/ml) with PAF C-16 (1 μg/ml) and anti-TNFα antibody (10 μg/ml) with PAF C-16 (1 μg/ml) (Test), before lysis and plating. The data is expressed in terms of percentage, where solvent control is considered as 100% survival and different treatment conditions are compared to it. The data represent median, interquartile and minimal and maximal values for four individual experiments performed in triplicates. Statistically significant (*p = 0.02) differences were found for ‘Test’ v 1 μg/ml PAF C-16 and ‘Test’ v Isotype control condition using Mann-Whitney test.