Fig. 2.

(A) Nyquist plots for an EIB for SEB, and mathematically fitted Nyquist plots. (B) Bode plots of |Z| versus f obtained from the EIB for SEB. EIB for SEB was developed using an anodic aluminum substrate and APTES. An anodic aluminum substrate with pores approximately 30 nm in diameter was treated with APTES. Anti-SEB was covalently immobilized on APES-SAMs deposited on the anodic aluminum substrate using glutaraldehyde. EIS of the EIB for SEB was performed at a biased potential of 0.1 V (vs. an Ag/AgCl reference electrode), in the absence or presence of 10 mg/mL SEB in 0.3% NaCl solution