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. 2020 Jun 10;8:420. doi: 10.3389/fcell.2020.00420

FIGURE 2.

FIGURE 2

Multiple sequence alignment of human ATG8 family members. We used Clustal Omega (Madeira et al., 2019) to generate a multiple sequence alignment of all the human ATG8 proteins (LC3A, LC3B, LC3C, LC3B2, GABARAP, GABARAPL1, GABARAPL2, and GABARAPL3), after retrieving the corresponding FASTA sequences from UniProt (Bateman, 2019). The secondary structure definition has been calculated using DSSP (Kabsch and Sander, 1983; Joosten et al., 2011), and as a reference an experimentally resolved structure of LC3B [PDB ID 3VTU (Rogov et al., 2013)]. The figure was generated using the ESPript 3.0 web server (Robert and Gouet, 2014) with default parameters for the calculation of the conservation scores and tuning the graphical representation of the alignment. Positions featuring highly conserved residues are colored with a blue background.