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. 2020 Jun 16;10:9715. doi: 10.1038/s41598-020-66808-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Purine alkaloid contents in different parts of ‘six and a bud’ tea shoots. (A) theobromine (TB), (B) caffeine (CAF), (C) theacrine (TCR), (D) total purine alkaloids (TPA). RYQT, ‘Ruyuan Qunti’; BFK, ‘Benifuuki’; BYC, ‘Baiyacha’. A. bud and first leaf; B. second leaf; C. third leaf; D. fourth leaf; E. fifth leaf; F. sixth leaf. The data are presented as the mean ± standard error of three independent analyses. Different letters above columns denote the existence of significant difference (p < 0.05).