Skip to main content
. 2020 Jun 16;11:3045. doi: 10.1038/s41467-020-16853-x

Fig. 1. Tax induces alternative splicing modifications independently of its transcriptional effects.

Fig. 1

a Genes regulated at the steady-state expression level and at the splicing level upon Tax expression in HEK cells. The significance thresholds were typically set to 10% for ∆PSI (differential percentage of spliced-in sequence) and 0.6 for log2-gene expression changes (p < 0.05, Fisher’s exact test), respectively. b Different alternative splicing events induced by Tax: alternative acceptor (acc), alternative donor (don), exon skipping (ES), multi-exon skipping (MES), and multi-exclusive exon skipping (MEx). c Validation of alternative splicing predictions by RT-PCR (using 35 cycles). The exon number is indicated in red. CD44 full variants (Ev*) were assessed using primers C13 and C12A (Supplementary Fig. 5). Representative image from three independent experiments is shown. d Exon-based hierarchical clustering. Kruskal–Wallis ANOVA (p < 0.05) was carried out with Mev4.0 software (http://www.tm4.org/) using the PSI values of exons that share similar regulations upon Tax and in clinical samples (EGAS00001001296). Only the most significant exon regulations are presented. Source data are provided as a Source Data file.