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. 2020 Jun 16;11:3045. doi: 10.1038/s41467-020-16853-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Relative occupancy of DDX17 at Tax-regulated genomic exons in cells that did or did not express Tax and knocked down or not with RELA-specific siRNA. b Relative gene expression levels of IL8, DDX17, and RELA in ATL2, C91PL, or HEK cells transiently transfected by pSG5M-Tax as compared uninfected MOLT4 cells. Tax mRNAs levels are expressed in arbitrary units (a.u.). c Alternative splicing modifications in the HTLV-1-infected cell lines ATL2 and C91PL as compared to those in the uninfected cell line MOLT4. Relative exon inclusion was measured as described in Fig. 3. d Relative RELA and DDX17 occupancies of regulated exons in ATL2 cells as compared to MOLT4 cells. Each occupancy of regulated exon by RELA and DDX17 is represented as a fold of that measured at its neighboring constitutive exon. Source data are provided as a Source Data file. Data are presented as the mean ± SEM values from biological replicates. Each black square represents a biological replicate. Statistical significance was determined with two-way ANOVA followed by Fisher’s LSD test (a, d) and two-tailed unpaired t-test (b, c) (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Exact p-values for Tax vs CTL (a): 0.0312 (CD44), 0.0337 (SEC31B), 0.0002 (CASK), 0.0171 (MYCBP2). Exact p-values for ATL2- and C91PL vs MOLT4 (b): 0.0001, 0.0004, 0.0003 and <0.0001, 0.0365, 0.0007 corresponding to IL8, DDX17, and RELA, respectively. For Tax expression, p = 0.0016 HEK + Tax vs ATL2. Exact p-values for ATL2- and C91PL vs MOLT4 (c): 0.0002, 0.0008, 0.0153, 0.0105 and 0.019, 0.0013, <0.0001, 0.11 for CD44, SEC31B, CASK, and MYCBP2 respectively. Exact p-values (d) for ATL2 vs MOLT4: qChIP-RELA < 0.0001 (CD44), 0.0003 (SEC31B), 0.0004 (CASK), 0.0029 (MYCBP2); qChIP-DDX17 0.0004 (CD44), 0.0211 (SEC31B), 0.0207 (CASK), 0.0184 (MYCBP2). Source data are provided as a Source Data file.