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. 2020 Jun 16;10:9695. doi: 10.1038/s41598-020-66559-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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WNT5a modulated the activity of multiple signaling pathways in OSE cells. (A) OSE cells were cultured and challenged with WNT5a for the times shown. Total cell protein was used to measure active and total CTNNB1 and phosphorylated and total CAMKII by western blot. Representative immunoblots show 3 samples per time point from three independent experiments. Quantitative analyses of active-CTNNB1/CTNNB1 and p-CAMKII/CAMKII protein levels for each time point in OSE are presented below the blots. Western blot for each protein is normalized to ACTB of their own blot (n = 3) and the representative image of ACTB belongs to active-CTNNB1 and p-CAMKII blots. Data are means ± SEM of three independent replicates. ANOVA with Dunnett’s post-test, **P < 0.01, indicates significant differences from control vs. WNT5a treatment. (B) Representative images of immunofluorescence staining for active-CTNNB1on OSE cells treated with WNT5a for 2 h (n = 3). Scale bar = 50 µm.