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. 2020 Jun 16;11:3067. doi: 10.1038/s41467-020-16918-x

Fig. 5. Liver HuR ablation reduces the levels of factors regulating lipid transport and ATP synthesis in HFD-fed mice.

Fig. 5

a Protein lysates prepared from the mouse livers (WT and cKO, n = 6) described in Fig. 3 were subjected to western blot analysis to assess the levels of proteins HuR, APOB-100, APOB-48, APOE, CYCS, NDUFB6, UQCRB, and TUBB. Blots were processed from parallel gels. b The density of the protein signals for APOB-100(B100), APOB-48 (B48), APOE, CYCS, NDUFB6, and UQCRB is represented as the means ± SD (WT and cKO, n = 6); significance is analyzed by two-tailed Mann–Whitney U test (*p < 0.05; **p < 0.01). Blank columns, WT; Black columns, cKO. c RNA prepared from livers described in a was subjected to RT-qPCR analysis to assess the levels of Apob, Apoe, Cycs, Ndufb6, and Uqcrb mRNAs (WT and cKO, n = 5). Data are the means ± SD (Blank columns, WT; Black columns, cKO); significance was assessed by using two-tailed Mann–Whitney U test (*p < 0.05; **p < 0.01). All the error bars are equivalent throughout the figure. Source data are provided as a Source Data file.