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. 2020 Jun 16;10:9722. doi: 10.1038/s41598-020-66636-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intrinsic Properties Characterization of PCa75 and antigen. (A) Differential Scanning Calorimetry melting profile of PCa75 (orange) and PCa62 (green). Heat capacity Cp (kcal/mol/°C) is plotted against the temperature (°C) (B) Isothermal Chemical Denaturation in GdnCl as monitored by change in fluorescence intensity ratio 350/330 nM for PCa75 (orange) and D) PCa62 (green). (C) Isothermal Chemical Denaturation in GdnCl as monitored by change in fluorescence intensity ratio 350/330 nM for the Fab of PCa75 (orange) and the Fab of PCa62 (green).