Table 2.
Thermal stability and Intrinsic Stability parameters (DSC and DSF). Thermal Stability Parameters determined from DSC (onset of unfolding and Fab domain unfolding Tm) and Intrinsic Stability Parameters from ICD experiments. ΔGu1, ΔGu2, c50 are the calculated parameters from 2-state and 3-state fitting of GdnCl induced denaturation curves generated in nano DSF experiment.
| mAb | Onset (DSC) (°C) | Tm (Fab): DSC (°C) | ΔGu1 (kJ/mol) | c50 [M] | ΔGu2 (kJ/mol) | c50 [M] |
|---|---|---|---|---|---|---|
| PCa75 | 52.9 | 61.8 | 24.3 | 1.8 | NA | NA |
| PCa62 | 59.3 | 75.5 | 63.5 | 1.9 | 37.2 | 2.9 |