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. 2020 Jun 10;11:514. doi: 10.3389/fgene.2020.00514

TABLE 4.

Summary of Kompetitive allele-specific PCR (KASP) assay for target traits.

SNP combinations Total genotypes in BC1F2 Rust score (in BC1F3)* LLS score (in BC1F3)† Oleic acid (%) (in BC1F3)¥
All six target SNPs 16 3–4 3–4 61.2–83.4
High oleic SNP 71 4–7 4–7 60.4–83.4
LLS and rust resistance associated SNPs 45 3–4 3–5 40.6–55.2
Null alleles for LLS and rust resistance and high oleic trait 211 5–8 5–8 38.4–52.6

*-Range of rust score at 35 days after inoculation (DAI), †-range of LLS score at 35 DAI, ¥-Range oleic acid content in selected genotypes based on presence of SNPs for target traits.