TABLE 2.
Ingredients for the preparation of Enzyme Solution.
| Chemical | Manufacturer | Catalog number | Quantities | Notes on preparation |
| MES, free acid | GoldBio, St. Louis, MO, United States | M-095-100 | 20 mM | Add Type I water to desired volume, pH with NaOH, filter sterilize or autoclave |
| D-Mannitol | Millipore Sigma, St. Louis, MO, United States | M1902 | 0.4 M | |
| KCl | Millipore Sigma | 1.04935 | 10 mM | |
| NaOH | Fisher Scientific, Waltham, MA, United States | S318-100 | pH to between 5.7 and 6.0 | |
| Cellulase R-10 | Duchefa Biochemie, Haarlem, Netherlands | C8001 | 1% (wt/vol) | Shortly before using add cellulase and macerozyme, and warm to 55°C for 10 min |
| Macerozyme R-10 | Duchefa Biochemie | M8002 | 0.25% (wt/vol) | |
| CaCl2 | Millipore Sigma | C8106 | 10 mM | Cool to room temperature, add CaCl2 and BSA |
| Bovine serum albumin | Millipore Sigma | A2153 | 0.1% (wt/vol) |